Micrococcus luteus: Difference between revisions
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'''''Micrococcus luteus''''' (''M. luteus''), is a [[Gram-positive bacteria]] that is most commonly found in mucous membranes such as the nasal cavities, the lining of the mouth etc. If we were to break down the word ''Micrococcus'', it would be as follows: Micro, for microscopic; ''coccus'' for the organism's spherical shape; ''luteus'' for "yellow". The bacteria is also found in dust, soil and the air that we breathe, and is part of the human skin flora. Although once ragarded as [[pathogen|non-pathogenic]], it is now considered an opportunistic pathogen, especially in immunocompromised patients. It is also responsible for [[nosocomial]] infections. As it is a commensal on the skin and nasal cavities, it is often overlooked as a source of clinical infection. | '''''Micrococcus luteus''''' (''M. luteus''), is a [[Gram-positive bacteria]] that is most commonly found in mucous membranes such as the nasal cavities, the lining of the mouth etc. If we were to break down the word ''Micrococcus'', it would be as follows: ''Micro'', for microscopic; ''coccus'' for the organism's spherical shape; ''luteus'' for "yellow". The bacteria is also found in dust, soil and the air that we breathe, and is part of the human skin flora. Although once ragarded as [[pathogen|non-pathogenic]], it is now considered an opportunistic pathogen, especially in immunocompromised patients. It is also responsible for [[nosocomial]] infections. As it is a commensal on the skin and nasal cavities, it is often overlooked as a source of clinical infection. | ||
''M. luteus'' (formerly ''Micrococcus lysodeikticus'') is of historical interest for the part it played in [[Alexander Fleming|Fleming]]’s discovery of [[lysozyme]], to which it is exquisitely sensitive<ref>Fleming A (1922) On a remarkable bacteriolytic substance found in secretions and tissues ''Proc Roy Soc B'' 93:306</ref><ref>Fleming A (1922) Observations on a bacteriolytic substance (Lysozyme) found in secretions and tissues ''Brit J. Exp Path'' 3:252</ref> | ''M. luteus'' (formerly ''Micrococcus lysodeikticus'') is of historical interest for the part it played in [[Alexander Fleming|Fleming]]’s discovery of [[lysozyme]], to which it is exquisitely sensitive<ref>Fleming A (1922) On a remarkable bacteriolytic substance found in secretions and tissues ''Proc Roy Soc B'' 93:306</ref><ref>Fleming A (1922) Observations on a bacteriolytic substance (Lysozyme) found in secretions and tissues ''Brit J. Exp Path'' 3:252</ref> | ||
Revision as of 04:38, 2 December 2010
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Micrococcus luteus (M. luteus), is a Gram-positive bacteria that is most commonly found in mucous membranes such as the nasal cavities, the lining of the mouth etc. If we were to break down the word Micrococcus, it would be as follows: Micro, for microscopic; coccus for the organism's spherical shape; luteus for "yellow". The bacteria is also found in dust, soil and the air that we breathe, and is part of the human skin flora. Although once ragarded as non-pathogenic, it is now considered an opportunistic pathogen, especially in immunocompromised patients. It is also responsible for nosocomial infections. As it is a commensal on the skin and nasal cavities, it is often overlooked as a source of clinical infection.
M. luteus (formerly Micrococcus lysodeikticus) is of historical interest for the part it played in Fleming’s discovery of lysozyme, to which it is exquisitely sensitive[1][2]
Genome structure
Cell structure and metabolism
It is a bacteria is an obligate aerobe which can also live in very low concentrations of carbon dioxide. Micrococcus luteus grows yellow round-shaped colonies. Its cell wall is made of peptidoglycans linked together by amino-acids which would explain its ability to absorb the dark-blue or violet stains. It is known to cause odors in humans because of its ability to break down sweat components.[3]
Ecology
M. luteus is mostly found in environments where the temperature is around 37 °C (98.6 °F) since this is the temperature at which its usual milieu is known to be (skin/nasal cavity). The bacteria grows also in beer and its lifetime is limited in soil. M. luteus is known for its growth in dairy products which facilitates its transmission to its host after consumption of milk for instance. This organism is able to grow on inorganic nitrogen and cannot synthesize acid from glucose in the presence of oxygen.[4] M. luteus can be used in the degradation of metals such as zinc, lead and nickel.[5]
M luteus has an unusual ability to tolerate and to use very toxic organic molecules as carbon sources, and metals. M luteus has been sequenced because these features are of particular importance for potential applications in bioremediation and biotechnology[6]- these two properties are essential to dealing with toxic wastes [7]
M. luteus has been found in contaminated soils, and is able to degrade phthalates,[8] hydrocarbons and olefinic compounds [9]
Pathology
Micrococcus is not considered as a pathogen but in individuals with a compromised immune system, such as newborn infants or patients with AIDS, M. luteus can cause skin infections that produce pruritic eruptions, sometimes with central ulceration, accompanied by severe itching. These symptoms are found most of the time in patients who have been admitted to hospital. The bacteria is transmitted by the intermediary of the medical staff who may have failed to wash their hands properly by going from one patient to another. The symptoms could start appearing after 48 hours within the facilities.
Application to Biotechnology
M. luteus has been used to study microbes’ susceptibility to lactoferrin, a glycoprotein. Lactoferrin exerts antibacterial activities by binding to the [lipomannan] of the cell wall of the microbe. The protein (mostly found in mammalian [exocrine] secretion) leads to the destruction of its host in certain species. For instance, in the Micrococcus genus, the glycoprotein is only effective in the M. luteus species but not for [M. radiophilus], [M. roseus] or [M. varians]. [10]
Lactoferrin is known for its iron-binding capacities. As iron is one of the factors promoting microbial viability and growth, its binding to lactoferrin stops the bactericidal effect. The amount of iron present for the lactoferrin to bind plays an important role in such activity; ferric salts can stop the antibacterial effect more than the ferrous salts.
Because M. luteus is part of the natural flora of the skin and mucous membranes, when a patient has a skin infection, this bacteria is not among the first pathogens that come to mind. Some tests are needed to confirm that M. luteus is indeed causing the symptoms. Often, M. luteus can be mistaken for Staphylococcus aureus, a bacteria that, just like his homologue, can cause nocosomial infections. However, M. luteus is coagulase negative where as S. aureus is coagulase positive and is a facultative anaerobe. S. aureus is also resistant to antibiotics, particularly bacitracin.
The Bacitracin susceptibility test is administered on M. luteus and S. aureus to differentiate one from another. A blood agar plate is prepared and separated in two sections. One side is where the M. luteus will be deposited by the use of a sterile swab and the other side is where the S. aureus will be deposited. A small disk of bacitracin is then placed on each side of the blood agar plate and left to rest for a few hours. When the plate is then studied, one can notice that the area around the bacitracin disk placed in the M. luteus side of the blood agar plate is cleared out due to its antibacterial effect on the Gram-positive bacteria. This area is known as the " zone of inhibition" thus it is susceptible to bacitracin. However, the contrary is observed for the S. aureus side of the plate since such bacteria is known to be antibacterial resistant. Bacitracin finds a certain difficulty to translate Gram-negative bacteria's mRNA.
Current Research
References
- ↑ Fleming A (1922) On a remarkable bacteriolytic substance found in secretions and tissues Proc Roy Soc B 93:306
- ↑ Fleming A (1922) Observations on a bacteriolytic substance (Lysozyme) found in secretions and tissues Brit J. Exp Path 3:252
- ↑ http://microbewiki.kenyon.edu/index.php/Micrococcus
- ↑ http://www.microbionet.com.au/mluteus.htm
- ↑ http://genome.jgi-psf.org/draft_microbes/miclu/miclu.home.html
- ↑ Micrococcus luteus NCTC 2665 Tree of Life
- ↑ Sandrin TR, Maier RM (2003) Impact of metals on the biodegradation of organic pollutants Environ Health Persp 111:1093-101
- ↑ Eaton RW (1982) Metabolism of dibutylphthalate and phthalate by Micrococcus sp. strain 12B J Bacteriol 151: 48–57
- ↑ Zhuang WQ et al. (2003) Importance of Gram-positive naphthalene-degrading bacteria in oil-contaminated tropical marine sediments. Lett Appl Microbiol 36: 251
- ↑ http://www3.interscience.wiley.com/cgi-bin/fulltext/119173366/main.html,ftx_abs