Induced pluripotent stem cells: Difference between revisions

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*Somatic cell nuclear transfer
*Somatic cell nuclear transfer
*Embryonic stem cell fusion
*Embryonic stem cell fusion
*Nuclear Reprogramming
*Nuclear reprogramming


==Nuclear Reprogramming==
==Nuclear reprogramming==
Based on previous studies, one research group set out to determine which genes are required to be expressed in order to generate/maintain embryonic stem cells.1  In a series of experiments it was determined that 4 factors are required to cause mouse fibroblasts to "dedifferentiate" into a stem-cell like state.
Based on previous studies, one research group set out to determine which genes are required to be expressed in order to generate/maintain embryonic stem cells.1  In a series of experiments it was determined that 4 factors are required to cause mouse fibroblasts to "dedifferentiate" into a stem-cell like state.
*Sox2
*Sox2
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*c-myc
*c-myc


==references==
==References==
1.  Takahashi K, Yamanaka S.  Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.  Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10.  PMID: 16904174 [PubMed - indexed for MEDLINE]
1.  Takahashi K, Yamanaka S.  Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.  Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10.  PMID: 16904174 [PubMed - indexed for MEDLINE]

Latest revision as of 18:23, 26 December 2007

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Introduction

Induced pluripotent stem cells (iPS cells) are a class of stem cells that are artificially derived from differentiated embryonic or adult tissues. Therapeutically, iPS cells hold the potential to be used to treat a patient with their own cells (i.e. bone marrow transplant, spinal cord injury repair).

Multiple strategies to generate iPS cells are being developed.

  • Somatic cell nuclear transfer
  • Embryonic stem cell fusion
  • Nuclear reprogramming

Nuclear reprogramming

Based on previous studies, one research group set out to determine which genes are required to be expressed in order to generate/maintain embryonic stem cells.1 In a series of experiments it was determined that 4 factors are required to cause mouse fibroblasts to "dedifferentiate" into a stem-cell like state.

  • Sox2
  • Klf4
  • Oct3/4
  • c-myc

References

1. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10. PMID: 16904174 [PubMed - indexed for MEDLINE]